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What are Peptides?

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~Dr Juice~

Jan 1, 1999
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Victoria B.C.
What are Peptides?

Peptides are short chains of amino acids linked together. If there are only two amino acids then the peptide is a dipeptide. Similarly there are tripeptides, tetrapeptides, and so on. If the number of amino acids in the chain reaches around ten or so, such substances are called polypeptides, while large polypeptides are called proteins. There is no particular agreed size at which a large polypeptide becomes a small protein, but generally polypeptides have molecular weights of a few thousand, while proteins have molecular weights of tens of thousands. Depending on which amino acids are involved, between seven and ten amino acids will add about 1000 to the molecular weight.

Peptides will help the muscle or soft tissue in this rebuilding healing process.

Muscle mass can be built quickly because the athlete can make small tears in a muscle and have it heal on a rapid schedule to continuously repeat the process – the end effect being increased muscle mass and reduced body fat in a shorter timeframe.

The bodybuilding community use peptides that are most effective in this second way as newer peptides don’t come with the side-effects of anabolic steroids.

Peptides will most likely come in a vial of white lyophilised solid powder which will require the user to dilute / reconstitute / mix with Bacteriostatic water also known as Bac water.

Sterile Water VS. Bacteriostatic Water for Peptides

What is bacteriostatic?
The term “Bacteriostatic” refers to a preservative called (benzyl alcohol) that is added to products to prolong there shelf life.

What is the difference between bacteriostatic water vs sterile water for injection?
Bacteriostatic water for injection has an extra preservative agent added into the water called benzyl alcohol (BnOH). Benzyl alcohol is a colorless liquid that has low toxicity, and low vapor pressure.

Sterile Water for injection is a single-dose sterile water that does not contain any bacteriostatic, antimicrobial agents, and added buffers.

How long does bacteriostatic water last once it is used?
Hospira recommends that once a vial of Bacteriostatic water is open the preservatives will prolong its life for up to 28 days. After 28 days the vial needs to be discarded. Other injectables without the bacteriostatic agent (single-dose vials) should be discarded after one single use.

  • Note: Please speak to your doctor before using Bacteriostatic Water or any injection products on adults and small children. Not for use in newborns and not to be used for inhalation.

What is the vial material made
The semi-rigid vial is fabricated from a specially formulated polyolefin. It is a copolymer of ethylene. The safety of the plastic has been confirmed by tests in animals according to USP biological standards for plastic containers. The container requires no vapor barrier to maintain the proper labeled volume.

What does bacteriostatic mean?
Bacteriostatic is a preservative called Benzyl Alcohol that is added to sterile water for injection to allow the solution to be reused for up to twenty eight days.

Many people wonder “what is the difference between sterile water, and bacteriostatic water?” The answer is quite easy to understand and can be explained easily. Sterile water, is water that is sterile and good for a 1 time injection. It does not include anything to prevent bacteria, so once it is penetrated it needs to be used entirely or disposed of. Bacteriostatic water is simply sterile water, with benzyl alcohol added to prevent bacteria growth in the water. Bacteriostatic water can be used for multiple injections spread over up to 30 days. Without the benzyl alcohol the water would not be safe for multi injection use. This is why it is very important to only use bacteriostatic water while doing the HCG diet. Bacteriostatic water is needed for ANY protocol of the diet, and if you substitute sterile water in its place you are risking infection.

Are peptides just for men?
A female looking to imitate her own natural frequent mini pulses of gh would do well to use CJC-1295 DAC, as it imitates her own pulses.

But can you stack it and make it more effective… the quick answer is YES!! the long answer is as follows

GHRP-6 is from the first generation of GHRPs and causes significant release of growth hormone by itself, due both to its suppression of somatostatin (an antagonist to GHRH) and stimulation of release of GH from the anterior pituitary. It does increase cortisol but not as significantly as say Hexarelin, it does though increase hunger and sadly unlike Ghrelin does not cause fat loss… not on its own, but only through GH release…but used in moderation with CJC-1295 DAC as above the results can be outstanding, seeing a marked increase in GH release, and the benefits that go along with that, of fat loss, improved skin tone, muscle tone, muscle recovery, even improved liver function, and increased igf levels leading to increased muscle growth, as well as helping any existing injuries by protecting tendons and joints.

Why isn't there as much in this vial as my other vials?
Each aliquot of peptide is deposited into each vial via machine. The vial is then stoppered and moved inside the lyophilizer. Once in the lyophilizer, the vial is then given a nitrogen flush to rid any contaminates. This is the phase that will determine the appearance of the contents.

Sometimes the water vapor can escape the vial where it goes from a solid to a gas without every going through a liquid phase. This will make the contents adhere to the sides of the vial leaving very little to see by the naked eye. Other times, depending on several other factors including temperature, the appearance can look like a solid puck, a few smaller pucks, a powder material, or even just a few specks, but the amount of actual material is the same.

When freeze drying products without any matrix (sucrose or mannitol for example) sometimes the residual water of hydration will cause a collapse via hydration of product before it fully dries. In other words, when the water from the ice crystals is removed what remains prior to secondary drying (when the chamber is warmed) can combine and solubilise the remaining material causing it to collapse. Sometimes it forms crystals and sometimes it forms films dependent upon how much there is local melting and what the temperature and vacuum levels are at the time of the collapse.

Very complicated but it tends to happen far less when sucrose for example is around since the sugar hydrogen bonds to the protein/peptide like the water did and thus less water remains bound as water of hydration

Do peptides come already mixed / reconstituted?
No peptides arrive already reconstituted. You should not purchase any peptide that has been mixed/reconstituted as once they have been mixed you should keep them refrigerated at all times.

What do I need to mix / reconstitute my peptides?
Peptides all need to be reconstituted / mixed with either Bacteriostatic Water / Acetic Acid or both. (see above - Sterile Water Vs Bacteristatic Water for Peptides)

Generally on the more complex and long chain peptides it is advisable to add a small amount of Acetic Acid (10 – 20%) first then top up to the desired level with Bacteriostatic Water.

Most simple peptides are fine to reconstitute with only Bacteriostatic Water. If you are keeping your Bacteriostatic Water in the fridge then allow to acclimatise nearer to room temperature before reconstituting with your peptide for better mixing.

These specialised waters keep the peptide from degrading, keeping the peptide fresh and bacteria free for longer periods.

My peptide looks cloudy?
Peptides can have widely varying solubility properties, depending largely on their primary sequence. While many peptides dissolve easily in bacteriostatic water (Containing 0.9% (9 mg/mL) of benzyl alcohol), some, especially those containing multiple hydrophobic amino acid residues, may not readily dissolve.

As a general procedure, we recommend first attempting to reconstitute peptides in bacteriostatic water. If solubility is still a problem, addition of a small amount of dilute (approximately 10%) aqueous acetic acid (for basic peptides) or aqueous ammonia (for acidic peptides) may facilitate dissolution of the peptide.
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